1. In the book Persian Fire by Tom Holland, the writer talks about a treaty between two city states in which they promise not to poison each other's water supplies (I'd have to take a look to get the details).
Anyway, biological warfare did exist in ancient times!
Albeit not with spies spreading the black death...
2. Well, that's the point of RTW, isn't it? Imean, what do you want to accomplish when you play the Aedui or Arverni in EB?
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