Yep. I'm in two Early hotseats with RR disabled, one Early hotseat with RR enabled (Double Jeopardy) and two Late hotseats with RR enabled. And when I play single player I do so with BYG, Limited Activities, Longer Assimilation and all the other bells and whistles. All you need to do is create a shortcut to the SS_setup file and use that each time you want to start the game - once you've run it the game starts automatically so it hardly takes any extra effort.
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