I would have to say the Fall of Rome.
It meant Europe would not be another China; it gave rise to multiple new kingdoms that competed with one another; it gave Europe a written heritage but did not dictate how Europeans interpreted it; it gave rise to struggles between Church and State; it undermined slavery and reoriented the European economy northwards; it transformed social relations, providing fertile ground for everything from feudalism to city-states; it united Germanic vigor and concepts of personal freedom with Greco-Roman language and learning; it not only allowed a new Europe to be born (in the age of Charlemagne and, much more strongly, in the 11th/12th centuries) but also gave it its educaton.
P.S. Can't wait for Barbarian Invasions!
P. P. S. Honourable mention goes to the Industrial Revolution.
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