I would agree with Phonics on this matter...
Unless Rome is no longer the Pope's capital, I don't think you can get it to riot (whatever the number of spies sent in, the number of building destroyed, or the size of the garrison)...
Even some of the nearest regions of the target faction can't be led to riot...
In my Hungarian campaign, with 5 spies in and three assassins destroying any PO and happiness building every turn, Vienna hasn't rioted at anytime leading to me capturing it when HRE declared war on me...
So to the OP, I would rather advocate the relocation of Vatican strategy, it has much a much better chance of success
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